What HPLC and mass spectrometry test in a research peptide, what a COA shows, and how to check a certificate is genuine.
Written by the Peptide Labs Research Team
Peptide testing in Australia usually means third-party laboratory analysis by HPLC (which measures purity) and mass spectrometry (which confirms identity), reported on a certificate of analysis (COA). A COA is a supplier-commissioned report, not a regulatory approval, so what matters is whether it is batch-specific, names the lab and method, and matches the vial in front of you.
| Test | What it measures | What it cannot tell you |
|---|---|---|
| HPLC | Purity: the main peak as a share of everything the detector saw | What the main peak actually is |
| Mass spectrometry | Molecular weight, to confirm the compound's identity | How much of the sample is that compound |
| Net peptide content | How much of the powder is peptide rather than salt and water | The purity of that peptide |
| Endotoxin and sterility | Bacterial contamination | Identity or purity |
HPLC and mass spectrometry are the standard pair. Endotoxin and sterility testing are separate tests, and most research peptide COAs do not include them.
A COA covers one batch only. It should show the batch number, the test date, the laboratory, the methods used, an HPLC chromatogram with the purity figure, and the mass spectrometry result with the observed mass next to the theoretical mass. A purity percentage on its own says little, which is why the guide to reading a peptide COA walks through each section.
A report that fails any of these is a reason to ask questions before relying on it. How to spot a scam peptide supplier covers the wider warning signs.
Yes. The COA library lists reports by batch, and each batch page shows the report issued by the testing laboratory, Janoshik Analytical for the bundled reports. A product that is still waiting on a report is listed as awaiting one rather than given a page that promises a certificate and shows nothing.
Look for a laboratory accredited to ISO/IEC 17025 (in Australia, NATA accredits laboratories to that standard) and ask whether it runs peptide HPLC and mass spectrometry on client samples. Availability varies, and many suppliers commission specialist overseas laboratories instead.
Peptide Labs supplies its products strictly for in-vitro laboratory research. They are not approved by the TGA for therapeutic use and are not for human or animal use. This article describes how analytical testing works and gives no dosing, preparation or use guidance.
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