Learn the correct technique for reconstituting lyophilised research peptides using bacteriostatic water for accurate, contamination-free preparations.
Written by the Peptide Labs Research Team
Lyophilised (freeze-dried) peptides must be reconstituted (dissolved in a liquid diluent) before use in research protocols. The standard diluent for most research peptides is bacteriostatic water (BAC water), which contains 0.9% benzyl alcohol to prevent microbial growth and enable multi-use of the vial.
Wipe down your work surface. Wash hands thoroughly or use gloves. Wipe the rubber stopper of both the peptide vial and BAC water vial with an alcohol wipe. Allow to air dry for 30 seconds.
Using a clean 29G insulin syringe, draw the desired volume of BAC water. For most peptides, 1–2mL is standard. For reference:
Insert the needle at an angle through the rubber stopper of the peptide vial. Slowly inject the BAC water down the side of the glass vial. Do not aim directly at the lyophilised powder cake, as this can degrade the peptide.
Gently swirl the vial in a circular motion. Do not shake or vortex. Allow the powder to dissolve completely. This may take 1–2 minutes. The resulting solution should be clear and colourless (some peptides may appear slightly coloured; this is normal for copper complexes like GHK-Cu).
Label the vial with the date of reconstitution. Store at 2–8°C (refrigerator). Most reconstituted peptides remain stable for up to 4 weeks when stored properly. Protect from light.
Converts vial strength and diluent volume into insulin-syringe units.
The reconstituted vial holds 200 units in total on a 100-unit-per-mL scale.
2 mL is more than the 0.5 mL syringe holds, so a single draw cannot take the full volume.
Arithmetic only, for laboratory measurement. It does not recommend a quantity, and nothing here is a protocol. Research use only.
| BAC Water Added | Peptide Amount | Concentration |
|---|---|---|
| 1mL | 10mg | 10mg/mL (10,000mcg/mL) |
| 2mL | 10mg | 5mg/mL (5,000mcg/mL) |
| 1mL | 5mg | 5mg/mL (5,000mcg/mL) |
Always label vials clearly with compound name, concentration, and date of reconstitution.
Disclaimer: All information is for educational purposes related to in-vitro laboratory research. Not intended as medical advice or guidance for human use.
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